2D DNA Nanonetworks

DNA nanotechnology is the fairly new field of research that deals with the construction of nanonetworks from smaller DNA components. Normally, dsDNA does not branch in vivo except as intermediate structures. However, it is possible to build 2D or 3D networks of DNA that can be used to position other molecules or molecular mechanical devices with high spatial precision. These nanonetworks can be used in nanorobotic devices, nanosensors, and nanomaterials.
Assembly of dsDNA is based on predictable and well-controlled base pair interactions. As a result, it is relatively easy to control the composition, organization and the binding properties of DNA-based materials making it an optimal material for all applications above. Thus far, most DNA networks have been constructed using tiles, or large pieces of DNA. However, we are attempting to use small, single-stranded oligonucleotides in order to facilitate faster self-assembly and smaller features within the structure. Smaller features would benefit certain applications of these nanostructures including the positioning of enzymatic cascades and nanocircuits. We are using commercially synthesized oligonucleotides in order to assemble 2D nanonetworks. Our results are first analyzed with agarose gel electrophoresis and then visualized using the Atomic Force Microscopy.